Gold Nanoparticle Photoaffinity Labels for Electron Microscopy
نویسندگان
چکیده
منابع مشابه
8-Azidoflavins as photoaffinity labels for flavoproteins.
8-Azidoflavins have been synthesized and their potential as photoaffinity labels for flavoproteins has been explored. They are very photolabile, and in aqueous media they react with solvent to yield 8-aminoflavins and 8-hydroxlaminoflavins as the main products. They fulfill the criteria expected of a good photoaffinity label, since they bind stoichiometrically at the flavin-binding site of flav...
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The photoreactivity of flavins has, surprisingly, never been used as a tool for the specific labelling of active centers. In contrast, an FAD analog modified in the adenine moiety, 8-azido-adenosine-FAD has been used for labelling studies with D-amino acid oxidase and glucose oxidase (1). In the past decade arylazides have been studied intensively as photoactivity labels (2), they react covalen...
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ژورنال
عنوان ژورنال: Microscopy and Microanalysis
سال: 2017
ISSN: 1431-9276,1435-8115
DOI: 10.1017/s1431927617010054